There was a small sample size (n=3-4), so we could not find significant differences between the two groups in the behavioral tests, including the corner test. In contrast, as I explained in the slide presentation at Friday's meeting, the trend increased. However, there was a significant difference in the infarct volume between the groups.
Pilot study will be also performed for 60 min occlusion. Here we showed the difference in between 30 and 40 min occlusion. In general corner test in SPAN protocol is on 7th day after MCAO occlusion. Here we did at 48h. Our purpose was to show the difference in between 30 min and 40 min occlusion and 48h infarction by TTC with some behavioral test.
I was wondering if we should try to standardize the lighting. We have recently started using lights with a red filter- the mice seem to be more active and less anxious in behavioral testing. Ali had put the information about what we are using in the google drive. Is anyone else using red lighting?
We have not been using red light routinely but I know it helps with behavior exams. It is not that hard. But there was a comment that it might be hard on the testing staff to be in that environment for a long time.
We did not use red lighting for the behavioral study yet. It does not make sense because we treated all groups (Control, stroke, and treatment) in the same environment. However, due to the circadian effect, animals are more active in the dark phase/cycle or dim light.
Corner test
Dr. Sheng,
There was a small sample size (n=3-4), so we could not find significant differences between the two groups in the behavioral tests, including the corner test. In contrast, as I explained in the slide presentation at Friday's meeting, the trend increased. However, there was a significant difference in the infarct volume between the groups.
Pilot protocol
Dr. Sheng,
Pilot study will be also performed for 60 min occlusion. Here we showed the difference in between 30 and 40 min occlusion. In general corner test in SPAN protocol is on 7th day after MCAO occlusion. Here we did at 48h. Our purpose was to show the difference in between 30 min and 40 min occlusion and 48h infarction by TTC with some behavioral test.
Augusta site pilot study: 60 vs 30 min (CCA-Open)
AUGUSTA site Pilot study: 60 vs. 30 min (CCA-Open)
Duke 30 min and 60 min Mouse CMAO
Duke 30 min and 120 min rat awake MCAO
I was wondering if we should…
I was wondering if we should try to standardize the lighting. We have recently started using lights with a red filter- the mice seem to be more active and less anxious in behavioral testing. Ali had put the information about what we are using in the google drive. Is anyone else using red lighting?
We have not been using red…
We have not been using red light routinely but I know it helps with behavior exams. It is not that hard. But there was a comment that it might be hard on the testing staff to be in that environment for a long time.
Red lighting....
We did not use red lighting for the behavioral study yet. It does not make sense because we treated all groups (Control, stroke, and treatment) in the same environment. However, due to the circadian effect, animals are more active in the dark phase/cycle or dim light.