From Augusta Site
Here is the sutures information for mice and rats recommended by the coordinating center. Augusta site followed the recommended sutures according to the weight of the animals (Mice and rats).
For Mice: (Doccol filaments)
- 6-0 fine MCAO suture L12 Re PK10 [Catalog No.: 602112PK10Re]
Silicone rubber-coated monofilament for MCAO model. Filament size 6-0, diameter 0.09-0.11 mm, length 20mm; diameter with coating 0.21 +/- 0.02 mm; coating length 1-2 mm, more suitable for skilled surgeons doing longer (>60 min) MCAO followed by reperfusion. Reusable. Pack of 10 filaments.
2. 6-0 medium A MCAO suture L12 Re PK10 [Catalog No.: 602212PK10Re]
Silicone rubber-coated monofilament for MCAO model. Filament size 6-0, diameter 0.09-0.11 mm, length 20 mm; diameter with coating 0.22 +/- 0.02 mm, suitable for MCAO models in animals within body weight range 25 +/- 2 grams; coating length 1-2 mm, more suitable for skilled surgeons doing longer (>60 min) MCAO followed by reperfusion. Reusable. Pack of 10 filaments.
3. 6-0 medium MCAO suture L12 reusable PK10 [Catalog No.: 602312PK10Re]
Silicone rubber-coated monofilament for MCAO model. Filament size 6-0, diameter 0.09-0.11 mm, length 20mm; diameter with coating 0.23 +/- 0.02 mm; coating length 1-2 mm, more suitable for skilled surgeons doing longer (>60 min) MCAO followed by reperfusion. Pack of 10 filaments. Reusable.
For RAT: (Doccol filaments)
CC recommended:
Up to 250 gm: 503123
250-280 gm: 503323
280 gm and over: 503523
- 5-0 medium MCAO suture L23 PK5Re [Catalog No.: 503123PK5Re]
Silicone rubber-coated monofilament for MCAO model. Filament size 5-0, diameter 0.14mm, length 30 mm; diameter with coating 0.31 +/- 0.02 mm, suitable for MCAO models in animals within body weight range 170 +/- 15 grams; coating length 2-3 mm, more suitable for skilled surgeons doing longer (>60 min) MCAO followed by reperfusion. Pack of 5 filaments. Reusable.
2. 5-0 large MCAO suture L23 PK5Re [Catalog No.: 503323PK5Re]
Silicone rubber-coated monofilament for MCAO model. Filament size 5-0, diameter 0.14mm, length 30 mm; diameter with coating 0.33 +/- 0.02 mm, suitable for MCAO models in animals within body weight range 200 +/- 15 grams; coating length 2-3 mm, more suitable for skilled surgeons doing longer (>60 min) MCAO followed by reperfusion. Pack of 5 filaments. Reusable.
3. 5-0 large MCAO suture B1 L23 PK5Re [Catalog No.: 503523PK5Re]
Silicone rubber-coated monofilament for MCAO model. Filament size 5-0, diameter 0.14mm, length 30 mm; diameter with coating 0.35 +/- 0.02 mm, suitable for MCAO models in animals within body weight range 250 +/- 15 grams; coating length 2-3 mm, more suitable for skilled surgeons doing longer (>60 min) MCAO followed by reperfusion. Pack of 5 filaments. Reusable.
We can select according to weight of the animals (mice and rats).
Suture information
From the Augusta site. We have used the following sutures according to the animal's (mice and rats) body weight recommended by the coordinating center in SPAN 1.0. For SPAN 2.0, we are also using these sutures again.
suture size
Dr. Khan,
Do you still remember how the filament sizes were decided in SPAN1? Here is what I found from DOCCOL. We used 0.37 for 90 min MCAO in the rats, which body weight were 250-280 grams.
Dr. Sheng I have explained…
Dr. Sheng
I have explained both mice' and rats' sutures in the forum. Please check carefully. However, here I have writing again for you as recommended by CC.
For RAT: (Doccol filaments)
CC recommended:
Up to 250 gm: 503123
250-280 gm: 503323
280 gm and over: 503523
suture
They are 0.02 mm less than what Doccol recommends.
Suture
We were used the same as I mentioned above and recommended by CC. We also found a consistent result in our stroke surgery.